| Do., 18.05.2000 | SC Fortuna Köln - Borussia Mönchengladbach | 1:2 |
| Do., 18.05.2000 | Chemnitzer FC - Stuttgarter Kickers | 1:1 |
| Do., 18.05.2000 | Alemannia Aachen - 1. FC Köln | 1:2 |
| Do., 18.05.2000 | FC Energie Cottbus - FC St. Pauli | 3:2 |
| Do., 18.05.2000 | FSV Mainz 05 - Tennis Borussia Berlin | 2:1 |
| Do., 18.05.2000 | Hannover 96 - SpVgg Greuther Fürth | 1:2 |
| Do., 18.05.2000 | Karlsruher SC - Rot-Weiß Oberhausen | 0:3 |
| Do., 18.05.2000 | SV Waldhof Mannheim - Kickers Offenbach | 2:3 |
| Do., 18.05.2000 | 1. FC Nürnberg - VfL Bochum | 0:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | 1. FC Köln | 32 | 18-8-6 | 62:34 | 28 | 62 |
| 2. | |  | VfL Bochum | 32 | 17-6-9 | 59:44 | 15 | 57 |
| 3. | |  | FC Energie Cottbus | 32 | 16-4-12 | 58:41 | 17 | 52 |
| 4. |  |  | Borussia Mönchengladbach | 32 | 13-12-7 | 53:40 | 13 | 51 |
| 5. |  |  | 1. FC Nürnberg | 32 | 13-10-9 | 49:44 | 5 | 49 |
| 6. | |  | Rot-Weiß Oberhausen | 32 | 11-12-9 | 39:33 | 6 | 45 |
| 7. | |  | Alemannia Aachen | 32 | 12-9-11 | 44:49 | -5 | 45 |
| 8. | |  | Hannover 96 | 32 | 12-7-13 | 52:51 | 1 | 43 |
| 9. | |  | FSV Mainz 05 | 32 | 10-12-10 | 38:36 | 2 | 42 |
| 10. | |  | SpVgg Greuther Fürth | 32 | 9-15-8 | 36:37 | -1 | 42 |
| 11. | |  | SV Waldhof Mannheim | 32 | 10-12-10 | 49:51 | -2 | 42 |
| 12. | |  | Chemnitzer FC | 32 | 10-10-12 | 40:44 | -4 | 40 |
| 13. | |  | Tennis Borussia Berlin | 32 | 10-10-12 | 41:46 | -5 | 40 |
| 14. | |  | FC St. Pauli | 32 | 8-14-10 | 33:38 | -5 | 38 |
| 15. | |  | Stuttgarter Kickers | 32 | 9-8-15 | 46:56 | -10 | 35 |
| 16. | |  | Kickers Offenbach | 32 | 8-11-13 | 33:52 | -19 | 35 |
| 17. | |  | SC Fortuna Köln | 32 | 6-11-15 | 31:48 | -17 | 29 |
| 18. | |  | Karlsruher SC | 32 | 5-11-16 | 34:53 | -19 | 26 |