| Do., 20.04.2000 | 1. FC Nürnberg - 1. FC Köln | 1:1 |
| Do., 20.04.2000 | Hannover 96 - Alemannia Aachen | 3:1 |
| Do., 20.04.2000 | FSV Mainz 05 - Rot-Weiß Oberhausen | 1:1 |
| Sa., 22.04.2000 | Borussia Mönchengladbach - Tennis Borussia Berlin | 4:2 |
| Sa., 22.04.2000 | SV Waldhof Mannheim - VfL Bochum | 4:2 |
| So., 23.04.2000 | Karlsruher SC - FC Energie Cottbus | 1:0 |
| So., 23.04.2000 | Kickers Offenbach - Stuttgarter Kickers | 0:4 |
| So., 23.04.2000 | SC Fortuna Köln - SpVgg Greuther Fürth | 0:1 |
| Mo., 24.04.2000 | Chemnitzer FC - FC St. Pauli | 1:3 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | 1. FC Köln | 28 | 15-8-5 | 52:27 | 25 | 53 |
| 2. | |  | VfL Bochum | 28 | 14-6-8 | 49:40 | 9 | 48 |
| 3. | |  | FC Energie Cottbus | 28 | 14-3-11 | 50:34 | 16 | 45 |
| 4. | |  | 1. FC Nürnberg | 28 | 12-9-7 | 44:40 | 4 | 45 |
| 5. | |  | Borussia Mönchengladbach | 28 | 11-11-6 | 47:35 | 12 | 44 |
| 6. |  |  | Hannover 96 | 28 | 11-7-10 | 45:42 | 3 | 40 |
| 7. | |  | SV Waldhof Mannheim | 28 | 9-12-7 | 42:41 | 1 | 39 |
| 8. |  |  | Alemannia Aachen | 28 | 10-9-9 | 37:44 | -7 | 39 |
| 9. | |  | Tennis Borussia Berlin | 28 | 9-10-9 | 35:39 | -4 | 37 |
| 10. | |  | Rot-Weiß Oberhausen | 28 | 8-11-9 | 32:31 | 1 | 35 |
| 11. | |  | SpVgg Greuther Fürth | 28 | 7-14-7 | 30:33 | -3 | 35 |
| 12. | |  | Chemnitzer FC | 28 | 9-8-11 | 36:40 | -4 | 35 |
| 13. | |  | FC St. Pauli | 28 | 7-13-8 | 29:32 | -3 | 34 |
| 14. | |  | FSV Mainz 05 | 28 | 7-12-9 | 33:33 | 0 | 33 |
| 15. | |  | Stuttgarter Kickers | 28 | 8-7-13 | 43:49 | -6 | 31 |
| 16. | |  | Kickers Offenbach | 28 | 6-11-11 | 27:42 | -15 | 29 |
| 17. | |  | SC Fortuna Köln | 28 | 5-11-12 | 25:40 | -15 | 26 |
| 18. | |  | Karlsruher SC | 28 | 4-10-14 | 31:45 | -14 | 22 |