| Do., 01.04.2010 | SV Wilhelmshaven - VFC Plauen | 1:3 |
| Fr., 02.04.2010 | Hertha BSC Berlin II - Tennis Borussia Berlin | 1:1 |
| Fr., 02.04.2010 | FC St. Pauli II - ZFC Meuselwitz | 0:2 |
| Sa., 03.04.2010 | Hallescher FC - Hamburger SV II | 0:1 |
| Sa., 03.04.2010 | VfB Lübeck - VfL Wolfsburg II | 1:5 |
| Sa., 03.04.2010 | Hannover 96 II - Türkiyemspor Berlin | 2:2 |
| So., 04.04.2010 | Chemnitzer FC - FC Oberneuland 1948 | 4:1 |
| So., 04.04.2010 | Goslarer SC 08 - 1. FC Magdeburg | 1:1 |
| So., 04.04.2010 | SV Babelsberg 03 - FC Hansa Rostock II | 2:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 26 | 17-7-2 | 40:13 | 27 | 58 |
| 2. |  |  | VfL Wolfsburg II | 24 | 14-6-4 | 40:13 | 27 | 48 |
| 3. | |  | Chemnitzer FC | 25 | 14-6-5 | 50:25 | 25 | 48 |
| 4. |  |  | Hallescher FC | 25 | 13-9-3 | 39:15 | 24 | 48 |
| 5. | |  | Hannover 96 II | 26 | 10-6-10 | 45:34 | 11 | 36 |
| 6. | |  | Hamburger SV II | 23 | 9-9-5 | 31:22 | 9 | 36 |
| 7. | |  | 1. FC Magdeburg | 26 | 9-8-9 | 43:29 | 14 | 35 |
| 8. | |  | VfB Lübeck | 26 | 10-5-11 | 34:38 | -4 | 35 |
| 9. | |  | VFC Plauen | 25 | 9-6-10 | 30:33 | -3 | 33 |
| 10. | |  | ZFC Meuselwitz | 24 | 8-9-7 | 28:31 | -3 | 33 |
| 11. | |  | Tennis Borussia Berlin | 27 | 8-8-11 | 25:39 | -14 | 32 |
| 12. | |  | FC Hansa Rostock II | 27 | 9-5-13 | 31:51 | -20 | 32 |
| 13. | |  | Türkiyemspor Berlin | 26 | 7-7-12 | 35:49 | -14 | 28 |
| 14. | |  | FC Oberneuland 1948 | 26 | 7-6-13 | 30:49 | -19 | 27 |
| 15. | |  | FC St. Pauli II | 24 | 6-8-10 | 26:38 | -12 | 26 |
| 16. | |  | Hertha BSC Berlin II | 25 | 6-7-12 | 37:47 | -10 | 25 |
| 17. | |  | SV Wilhelmshaven | 23 | 5-7-11 | 27:43 | -16 | 22 |
| 18. | |  | Goslarer SC 08 | 24 | 2-7-15 | 22:44 | -22 | 13 |